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Anti-clastogenic activity of two
structurally related pterocarpans purified from Bituminaria bituminosa
in cultured human lymphocytes.
Maurich
T, Pistelli
L, Turchi
G.
Mutagenesis and Biochemistry in Somatic Cells Unit,
IBF CNR, Via G. Moruzzi 1, Pisa, Italy.
Plant-derived isoflavones
are currently receiving much attention because of their
phyto-estrogenic, antioxidant, anti-mutagenic, and anti-tumor
activities. In this study we have evaluated the clastogenic and
anti-clastogenic activities in human lymphocytes of two structurally
related pterocarpans, iso-flavonoid derivatives, termed erybraedin C and
bitucarpin A, recently purified from Bituminaria bituminosa and
chemically characterized. Mitomycin C (MMC) and the radio-mimetic
bleomycin (BL) were used as reference clastogens. The end point studied
was micronucleus formation. The results obtained in this study indicate
that erybraedin C and bitucarpin A, when assayed alone, do not affect
either the mitotic index or the cell-proliferation index of human
lymphocytes. Interestingly, both compounds appear to be non-clastogenic
in the range of concentrations used. In contrast, both substances seem
to affect significantly the clastogenic effects induced by BL and MMC. A
1-h pre-exposition of the cell culture to erybraedin C was necessary to
display its anti-clastogenic potential against BL, whereas bitucarpin A
was inactive in this respect, with a structure-activity relationship. In
contrast, the clastogenic activity of MMC was significantly reduced by
both erybraedin C and bitucarpin A, using either a pre-incubation
schedule or simultaneous treatment. These results suggest that the
protective effects displayed by the two anti-clastogenic compounds
against MMC could be due to the induction or inhibition of cellular
reductive metabolic enzymes.
PMID: 15238232 [PubMed - indexed for
MEDLINE]
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