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Purification and characterization of allergens
from Xanthium strumarium pollen.
Jaggi
KS, Gangal
SV.
CSIR Centre for Biochemicals, G.T.B. Marg, Delhi
University Campus, India.
The allergenic components present in
whole pollen extract of Xanthium strumarium were isolated by sequential
ammonium sulphate precipitation, DEAE Sephadex A50 chromatography and
gel filtration. The techniques of RAST inhibition and skin test were
utilized to check the allergenicity of fractionated proteins revealing
the presence of Xan Ib and Xan VIa as the important allergenic
components. Xan Ib was found to be devoid of carbohydrate and had a
molecular weight of 103,000 daltons. Xan VIa was a glycoprotein of
molecular weight 17,000 daltons. The carbohydrate moiety of Xan VIa was
found to be associated with allergenicity. The characteristic pattern of
whole pollen extract on CIE and TLIEF showed 36 and 21 protein bands,
respectively. The use of FPLC in isolation of partially purified
allergens from Xanthium is discussed.
PMID: 3441253 [PubMed -
indexed for MEDLINE]
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