 About Entrez
NCBI Toolbar
Text Version
Entrez PubMed
Overview
Help | FAQ
Tutorials
New/Noteworthy
E-Utilities
PubMed Services
Journals Database
MeSH Database
Single Citation Matcher
Batch Citation Matcher
Clinical Queries
Special Queries
LinkOut
My
NCBI
Related
Resources
Order Documents
NLM
Mobile
NLM Catalog
NLM
Gateway
TOXNET
Consumer
Health
Clinical Alerts
ClinicalTrials.gov
PubMed
Central |
|
| Display Show |
 |
|
|
-
First report of a glutamine-rich
antifungal peptide with immunomodulatory and antiproliferative
activities from family Amaryllidaceae.
Chu
KT, Ng
TB.
Department of Biochemistry, Faculty of Medicine, The
Chinese University of Hong Kong, Hong Kong, China.
This
represents the first report of purification of a glutamine-rich
antifungal peptide from family Amarylliaceace. The peptide, designated
as nartazin, was purified from the bulbs of the Chinese daffodil
Narcissus tazetta var. chinensis by means of ion-exchange chromatography
and affinity chromatography. Its molecular mass was 7.1kDa, as
determined by SDS-PAGE and gel filtration. Nartazin stimulated
proliferation of mouse splenocytes and bone marrow cells but inhibited
proliferation of leukemia L1210 cells. It also inhibited translation in
a cell-free rabbit reticulocyte lysate system. The sequence of its first
20 N-terminal residues was characterized by an abundance of glutamine.
The peptide possessed antifungal activity on four phytopathogenic fungi.
Its activity was retained after incubation with bovine trypsin and
chymotrypsin (enzyme: substrate ratio 1:10 w/w) at 37 degrees C for 1h
but was attenuated after treatment with proteinase K. The data revealed
its pronounced resistance to proteolytic digestion.
PMID:
15522215 [PubMed - indexed for MEDLINE]
| Display Show |
| |